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Metformin hydrochloride

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产品编号 T0740Cas号 1115-70-4
别名 盐酸二甲双胍, Metformin HCl, 1,1-Dimethylbiguanide hydrochloride, 1, 1-Dimethylbiguanide hydrochloride

Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) 是一种 AMPK 激活剂,具有血脑屏障渗透性。Metformin hydrochloride 可通过提高胰岛素敏感性和减少肠道对葡萄糖的吸收来改善血糖控制,常用于 2 型糖尿病的研究。

Metformin hydrochloride

Metformin hydrochloride

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纯度: 99.81%
产品编号 T0740 别名 盐酸二甲双胍, Metformin HCl, 1,1-Dimethylbiguanide hydrochloride, 1, 1-Dimethylbiguanide hydrochlorideCas号 1115-70-4

Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) 是一种 AMPK 激活剂,具有血脑屏障渗透性。Metformin hydrochloride 可通过提高胰岛素敏感性和减少肠道对葡萄糖的吸收来改善血糖控制,常用于 2 型糖尿病的研究。

规格价格库存数量
50 mg¥ 168现货
100 mg¥ 233现货
500 mg¥ 536现货
1 g¥ 728现货
5 g¥ 2,160现货
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产品介绍

生物活性
产品描述
Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) is an AMPK activator with blood-brain barrier permeability. Metformin hydrochloride may improve glycemic control by increasing insulin sensitivity and decreasing intestinal glucose uptake, and is commonly used in type 2 diabetes research.
体外活性
方法: 卵巢癌细胞 A2780 和 SKOV3 用 Metformin hydrochloride (0.001-50 mM) 处理 24-48 h,使用 MTS 方法检测细胞活力。
结果: 微摩尔浓度的 Metformin 在统计学上不会降低 A2780 或 SKOV3 细胞系的生存能力。在 48 h 时,毫摩尔浓度会导致细胞死亡。[1]
方法: 人结直肠癌细胞 HCT29 用 Metformin hydrochloride (0.6 mM) 处理 90 h,使用 wound healing assay 和 chamber invasion assay 检测细胞运动情况。
结果: Metformin 抑制 HCT29 细胞的迁移和侵袭。Metformin 降低肿瘤细胞的运动能力。[2]
体内活性
方法: 为建立 Metformin 诱导腹泻的模型,将 Metformin hydrochloride (125-500 mg/kg) 口服给药给健康和糖尿病肥胖 db/db 的 C57BL/6J 小鼠,每天两次,持续十三天。
结果: 1000 mg/kg/天的 Metformin 显著增加了粪便水分含量。尽管在健康 C57BL/6J 小鼠中未观察到腹泻症状,但相同剂量的 Metformin 在糖尿病肥胖 db/db 小鼠中诱导严重腹泻。[3]
方法: 为研究在辐射损伤中的保护作用,将 Metformin hydrochloride (200 mg/kg,每天一次持续三天) 口服给药给 BALB/c 小鼠,随后暴露于 6-8 Gy 的 γ 射线。
结果: 在暴露于辐射前给药时,Metformin 可以延长暴露于 8 Gy-TBI 的小鼠的生存期,并提高暴露于 6 Gy-TBI 小鼠的生存率。Metformin 预处理可以减轻辐射损伤。[4]
细胞实验
Hepatocytes were isolated from male Sprague Dawley (SD) rats by collagenase digestion. For the AMPK assay, cells were seeded in six-well plates at 1.5 × 10^6 cells/well in DMEM containing 100 U/ml penicillin, 100 μg/ml streptomycin, 10% FBS, 100 nM insulin, 100 nM dexamethasone, and 5 μg/ml transferrin for 4 hours. Cells were then cultured in serum-free DMEM for 16 hours followed by treatment for 1 hour or 7 hours with control medium, 5-aminoimidazole carboxamide riboside (AICAR), or metformin at concentrations indicated. For a 39-hour treatment, cells for both control and metformin (10 or 20 μM) groups were cultured in DMEM plus 5% FBS and 100 nM insulin, and the fresh control and metformin-containing medium were replaced every 12 hours (last medium change was 3 hours before harvest). After treatment, the cells were directly lysed in digitonin-containing and phosphatase inhibitor–containing buffer A, followed by precipitation with ammonium sulfate at 35% saturation. AMPK activity was determined by measurement of phosphorylation of a synthetic peptide substrate, SAMS (HMRSAMSGLHLVKRR). For ACC assay, the 35% ammonium sulfate precipitate from digitonin-lysed hepatocytes (4 μg each) was used for determination of ACC activity via 14CO2 fixation in the presence of 20 mM citrate as done previously. For fatty acid oxidation, the oxidation of 14C-oleate to acid-soluble products was performed as done previously, but in medium M199 in the absence of albumin [1].
动物实验
Oral gavage was used to administer 1 ml of metformin (100 mg/ml) or water alone to male SD rats (300–350 g, n = 7–8). Rats were treated once or twice a day for 5 days. Rats were starved for 20 hours and then re-fed for 2 hours before the final dose; 4 hours after the final dose, the animals were anesthetized and livers rapidly removed by freeze clamping followed by blood withdrawal. RNA was prepared from the freeze-clamped liver by RNA isolation reagent. Nuclear extracts were prepared from a pool of seven rat livers. Glucose levels were determined using the standard glucose oxidase assay kit; β-hydroxybutyrate concentrations were assayed by measuring the reduction of NAD to NADH with a standard assay kit. FFA levels were measured with the assay kit [1]. MCF10A-ER-Src cells (5 × 10^6) were injected into the right flank of 18 female nu/nu mice, all of which developed tumors in 10 d with a size of ~100 mm^3. The mice were randomly distributed into six groups (three mice/group) that were untreated or treated by intratumoral injections every 5 d (four cycles) with 1 mg/kg or 4 mg/kg doxorubicin, 200 μg/mL metformin (diluted in the drinking water), or the combination. In another experiment, LNCaP and DU145 prostate cancer cells (5 × 10^6) were injected into the right flank of 12 female nu/nu mice, all of which developed tumors in 10 d with a size of ~75 mm^3. The mice were randomly distributed into four groups that were untreated or treated by intratumoral injections every 5 d (four cycles) with 4 mg/kg doxorubicin and/or 200 μg/mL metformin. In another experiment, A375 and MDA-MB-435 melanoma cells (7 × 10^6) were injected into the right flank of 12 female nu/nu mice, all of which developed tumors in 10 d with a size of ~50 mm3. The mice were randomly distributed into four groups that were untreated or treated by intratumoral injections every 5 d (four cycles) with 10 mg/kg cisplatin and/or 200 μg/mL metformin.Finally, SNU-449 liver cancer cells (10^7) were injected into the right flank of 12 female nu/nu mice, all of which developed tumors in 10 d with a size of ~50 mm^3. The mice were randomly distributed into four groups that were untreated or treated by intratumoral injections every 5 d (four cycles) with 10 mg/kg cisplatin and/or 200 μg/mL metformin. Tumor volume (mean ± SD) was measured at various times after the initial injection [3].
别名盐酸二甲双胍, Metformin HCl, 1,1-Dimethylbiguanide hydrochloride, 1, 1-Dimethylbiguanide hydrochloride
化学信息
分子量165.63
分子式C4H12ClN5
CAS No.1115-70-4
SmilesCl.CN(C)C(=N)NC(N)=N
密度1.36. Temperature:20.1 °C.
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
H2O: 193.21 mM
DMSO: 15 mg/mL (90.56 mM), Sonication is recommended.
溶液配制表
1mg5mg10mg50mg
1 mM6.0376 mL30.1878 mL60.3755 mL301.8777 mL
5 mM1.2075 mL6.0376 mL12.0751 mL60.3755 mL
10 mM0.6038 mL3.0188 mL6.0376 mL30.1878 mL
20 mM0.3019 mL1.5094 mL3.0188 mL15.0939 mL
50 mM0.1208 mL0.6038 mL1.2075 mL6.0376 mL
1mg5mg10mg50mg
100 mM0.0604 mL0.3019 mL0.6038 mL3.0188 mL

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  • 稀释 计算器
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体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

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